dGH in-Site™ CAR-T Probe Kit
Track the sequence, location and orientation of key CAR-T genomic loci.
With our dGH in-Site™ CAR-T Kit, scientists can track the impact of cellular engineering on TRAC and B2M loci. The kit can also be expanded to 3- or 4-plex with the addition of custom dGH probes.
What is dGH?
directional Genomic Hybridization (dGH™) in-Site assays provide the most detailed and sensitive analysis of targeted genome edits available. In a single test, dGH can track the sequence, location and orientation of your gene-editing outcomes so you can see what is not visible using FISH or BAC FISH. dGH provides a limit of detection that is superior to FISH and G-banding. In addition, single-cell dGH data enables detection of low-level abnormalities missed in sequencing data from pooled DNA samples.
Key features of dGH™ technology:
- Cell preparation yields single-stranded metaphase chromosomes
- Single-stranded fluorescent probes provide direct visualization of editing outcomes including orientation and balanced/unbalanced structural variants
- Ultra-high resolution, as low as 2 Kb
- Lower limit of detection than FISH or BAC FISH
Learn more about our dGH™ technology →
Key benefits of the CAR-T probe kit:
- Gain novel, single-cell insights from direct visualization of DNA sequence, location and orientation
- High signal-to-noise ratio boosts scoring confidence and efficiency
- Provides data orthogonal and complementary to sequencing and G-banding
- Works with standard cytogenetic equipment and workflows
- Expert support from KromaTiD scientists on sample processing and image analysis
Use of the CAR-T probe kit requires specific hardware. Please download the requirements below:
dGH in-Site™ CAR-T Kit applied to control cell line showing TRAC (TexRed) and B2M (6-FAM) signals.
dGH in-Site™ CAR-T TRAC and B2M ideogram detailing probe loci target in homolog chromosomes.
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| Product | Product Contents | Price | Quantity | Add to Cart |
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dGH in-Site™ CAR-T Probe Kit 1
Track edits to CAR loci, pre- and post-genome editing.
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dGH™ Probe TRAC TexRed
dGH™ Probe B2M 6-FAM/Spectrum Green dGH™ Standard Hybridization Buffer |
$1,500.00
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dGH™ Cell Prep Additive - 250µl
Enough reagent to culture 8 samples in triplicate T25 flasks or 4 samples in triplicate T75 flasks |
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$1,000.00
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dGH™ Standard Hybridization Buffer
(10 Assays) |
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$10.00
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Colcemid™ solution 2.5 mL
Demecolcine solution 2.5 mL (Synonyms: Colcemid™ solution, N-Deacetyl-N-methylcolchicine solution)
Price per unit $10.00 |
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$10.00
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dGH in-Site™ Training: Basics of Image Analysis
dGH in-Site Kit Analysis by Kromatid / White Glove Training |
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$500.00
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dGH in-Site™ CAR-T Probe Kit 1 Bundle
Track edits to CAR loci, pre- and post-genome editing. Contains additional reagents required to prepare cells for dGH probe hybridization. |
dGH™ Probe TRAC TexRed
dGH™ Probe B2M 6-FAM/Spectrum Green dGH™ Standard Hybridization Buffer dGH™ Cell Prep Reagent 250 µL Demecolcine Solution 2.5 ml dGH™ Image Analysis Support |
$2,799.00
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