Pinpoint FISH - KromaTiD

What is Pinpoint FISH?

Pinpoint FISH (PPF) is a synthetic oligonucleotide based FISH assay designed to provide the highest resolution, lowest background, and lowest limit of detection available. By modifying the hybridization conditions and eliminating strand degradation steps, KromaTiD probes can be used in standard FISH assays, delivering improved specificity and hybridization kinetics.

PPF allows researchers to expand beyond the capabilities of conventional FISH probes to detect smaller targets and design ultra high specificity tests.

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kromatid assay

A lymphoblast cell line (GM15510) was fixative and probed with KromaTid p53 probe yellow.

RESOURCES

Product Brochure

MSDS

ADDITIONAL PRODUCTS

dGH SCREEN

dGH in-Site

G-Banding

Pinpoint FISH Specifications

Any FISH Application

KromaTiD offers genome wide, custom Pinpoint FISH assays designed to meet your research goals. Using our proprietary design package, KromaTiD provides assays optimized for detecting. Pinpoint FISH can be used for any conventional FISH applications including:

Deletions
Amplifications & CNVs
Break Aparts (Gene LOF)
Fusion Gene Detection

Requirements

KromaTiD has designed the Pinpoint FISH platform to minimize adoption costs and training. For labs that currently run FISH, Pinpoint FISH assays will work with your established samples, workflow and imaging systems without any capital expenditures. Pinpoint FISH is fully compatible with:

Standard FISH Equipment
Standard FISH Conditions
BAC Probes

Sample Requirements

For labs that don’t run FISH, but are interested in the high-resolution target data that only Pinpoint FISH can provide, KromaTiD’s service division can perform projects ranging from single sample analysis to full library screening, all we need from you is a sample. Sample requirements include:

FFPE
Fixed Cell Pellet
Any FISH Sample
Published Genome

Pinpoint FISH Benefits

KromaTiD offers reagents and kits for both inversion detection and Pinpoint FISH applications. Stock chromatid paints, chromosome paints and gene specific assays for inversion screening and discovery, biodosimetry analysis, and known inversion detection (including research-use-only tests for inversion-caused diseases like leukemia and thyroid cancer) are available in 10 and 50 assay kits. New paints and assays are frequently added to the catalog.

When compared to typical FISH methods, Pinpoint FISH from KromaTiD offers:

Better Performance

3x the relative signal; 3x the resolution
6x the signal-to-noise ratio; 50% lower background

More Flexibility

Pinpoint probes can be designed for a wider range of targets and target sizes
By design, Pinpoint FISH assays can easily detect mutations with variable breakpoints

Easier Use

Free of repetitive sequences by design, no blocking required
Robust performance and consistent hybridization quality

Custom Assays

KromaTiD can design and produce fully custom probes or assays for any target in the genome. Our custom assays are designed from the genome up to solve your individual research challenges.