Edit Integrity and Transgene Mapping

KROMASURE™ InSite ensures Edit Site Integrity throughout the entire cell and gene therapy workflow by providing precise characterization of genomic edits. Whether analyzing a single locus or multiple edit sites.

InSite detects ~2KB transgene insertions and provides a comprehensive view of copy number, transgene orientation, and off-target effects, complementing sequencing data for a robust genome integrity assessment.

For Starting Material Analysis including strucural characterization of the genome and comparitive reporting of cells during multiple passages to measure genomic stability over time. 

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Cell and Gene Therapy Workflow

Throughout the entire cell and gene therapy process, from R&D to the clinic, characterization of the genome is a critical component of ensuring the edit integrity of each gene editing platform. Whether the process involves a single locus or multiple edit sites, KROMASURE InSite can detect small structural variants and transgene inserts, providing confidence for edit integrity.

Utilizing proprietary Advanced Fluorescent Hybridization technology (dGH) KROMASURE Insite provides direct visualization of:

  • Structural variants on a single cell basis
  • Rearrangements including translocations
  • Inversions
  • Copy number variants 
Customized assays are developed to your specific target sites and provide additional orthogonal single-cell data to add to the data packages from other techniques such as sequencing.
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Edit site and transgene mapping

  • Exceptional lower limit of detection (LLOD) translates to ~ 2KB transgene
    fluorescent detection. ​ 
  • Probes are universal and will work in metaphase dGH, metaphase FISH,
    and interphase FISH assays. 
  • Off-target complex chromosomal abnormalities (such as dicentric formation and chromothrypsis) are tracked across the genome along
    with the side specific targeted edit site structural variants. 
  • Can be multiplexed with other locus-specific probes and/or chromosome
    paints.

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What it detects

  • Orientation of transgene integration​.
  • Chromosomal structural variants involving the integration site or other
    targets of interest​.
  • Insertional copy number

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KromaSure InSite

KROMASURE™ InSite

KROMASURE™ InSite offers access to our proprietary solution dGH that gives critical insights into edit site integrity and transgene mapping. 

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