Edit Integrity and Transgene Mapping

KROMASURE™ PinPoint™ is a proprietary solution designed to deliver high-throughput, single-cell-level analysis of transgene integration. Optimized for modern genomic research, PinPoint detects integration events as small as 2kb, setting a new benchmark for precision in edit integrity analysis.

While sequencing has long been the standard for assessing insertional copy number in cell and gene therapy, it provides only an average from pooled cell populations and algorithmic estimates. By analyzing individual cells, developers can uncover the true distribution of integrations - often highly variable from cell to cell.

With PinPoint you can directly visualize transgene integrations in thousands of cells. This gives developers expanded capability into how their platform behaves at a cellular level, helping them understand transgene distribution with far greater precision.

Applications:

  • Measure Genomic Integrity
  • Assess Edit Integrity
  • Map Transgene Insertion
  • Support Regulatory Compliance
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Core Capabilities

  • Characterize Small Target Integrations Detects transgenes and genomic targets as small as 2–10kb with high specificity.
  • Single-Cell Resolution Measures copy number and modal distribution of integration events at the chromosome and cell level.
  • Transgene Enumeration Accurately quantifies the number of transgene copies per nucleus.
  • Deletion Analysis Identifies deleted genetic material, beyond just functional knockouts.
  • Flexible Sample Compatibility Works with hepatocytes or any interphase cell, eliminating the need for metaphase preparation.

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Insertional Copy Number Insights

  • Precise Chromosomal Mapping
    Tracks where transgenes insert in the genome, down to individual chromosomes.
  • High-Throughput Statistical Power
    Analyzes thousands of nuclei for robust, data-driven conclusions.
  • Risk Profiling & Compliance
    Supports regulatory needs by building a solid foundation for risk assessment.
  • Modal Distribution
    Understands how integration events vary across cells for population-wide clarity.
  • Aneuploidy Detection
    Detects monosomy and accurately measures ploidy (chromosome copy number variation).
  • Integrated Data Analysis
    Fully compatible with KROMASURE™ InSite for comprehensive, orthogonal data interpretation.

KROMASURE Complete Genomic Integrity Graphic
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KROMASURE™ PinPoint

KROMASURE™ PinPoint offers insight into insertional copy number with modal distribution. Learn more!

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