Genomic Integrity G-Banding | Advanced Karyotyping Solutions

In Cell and Gene Therapy applications, cells are undergoing significant genomic manipulation during the gene editing process. This is why KROMATID developed KROMASURE™ KBand, a fit-for-purpose method of genomic integrity G-Banding.

For Starting Material Analysis the process begins with unbiased G-Band karyotyping, measuring both donor and treated cells. This method provides valuable insights into genomic structural variants related to editing and cell product expansion.

Then our team of experts compares this data to donor cells in our Genomic Integrity Pair-Wise Comparison Report, calculating statistical significance of the differences. For developers needing resolution beyond the ~10 mb provided by G-Banding, KROMATID offers other Genomic Integrity Assays in our proprietary KROMASURE™ platform, providing at least two orders of magnitude improved resolution for structural variant detection.

KBand: Genomic Integrity G-Banding

Robust G-Banding Process with statistically benchmarked treated vs reference cells
Detect Genetic drift: Insightful identification of chromosomal changes over time
Assess up to 500 cells for rare genetic events, surpassing the standard 20
cell analysis
GLP and GMP services for confidence in data for inclusion in regulatory filings

What it detects

Detects large SVs including inversions, translocations and monosomies
Monitor for sub-clonal outgrowth, instability and chromothripsis
A measurement of the SV variance from Donor to Product regardless of causation.
Lower limit of SV frequency determined by number of cells analyzed
Orthogonal to molecular genomic methods such as NGS, dPCR and dGH

Benefits

Enhanced Risk Assessment:
Identify rare events (e.g., 1% of cells with 100 aberrations).
Increased Confidence in Results:
Analyze up to 500 cells per batch for higher accuracy.
Specialized Expert Analysis:
Delivering differentiated reporting with actionable insights.
Statistically Robust Summaries:
Summarizing outcomes to support data-driven decisions.
Sub-clonal Detection:
Unveiling low-prevalence genetic variations.
Comprehensive Comparative Analysis:
Rolling up changes between pre-treatment, post-treatment, and reference samples.

Integrity G-Banding, Advanced Karyotyping and Starting Material Analysis

KBand: Genomic Integrity G-Banding

What it detects

Product Solutions Roadmap

Benefits

Direct Genomic Visualization

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