KROMASURE™ for Starting Material Analysis: Setting the Foundation for Safer, Smarter Therapies

In cell and gene therapy development, everything begins with the starting material — the raw cellular input that ultimately becomes the therapeutic product. Whether you're working with patient-derived cells, donor cells, or engineered lines, the genomic integrity of your starting material directly impacts product quality, safety, and regulatory success.

The KROMASURE™ Platform provides a powerful suite of tools for deep, single-cell structural and copy number analysis of starting material, delivering a critical layer of insight before manufacturing even begins. By identifying genomic abnormalities early, developers can make better decisions, reduce downstream risk, and build a stronger foundation for the entire product lifecycle.


Why Starting Material Genomics Matter

Starting material isn't just the beginning — it's the blueprint. Structural genomic issues like chromosomal translocations, aneuploidy, or undetected deletions can:

  • Compromise cell function or viability

  • Skew experimental outcomes

  • Undermine gene editing precision

  • Raise red flags during regulatory review

Yet traditional methods like karyotyping or bulk sequencing miss low-frequency and cell-specific events, leaving blind spots in the very cells you're building on.


KROMASURE™: Single-Cell Clarity from Day One

The KROMASURE™ Platform delivers non-metaphase, high-resolution genomic insight at the single-cell level, making it ideal for analyzing diverse starting material types, including:

  • Hematopoietic stem cells

  • T cells

  • iPSCs

  • Primary hepatocytes

  • Immortalized lines

With the ability to assess thousands of cells in a single run, KROMASURE™ gives you a statistically robust understanding of your starting population's structural stability, ploidy, and overall genomic integrity.


Key Benefits of KROMASURE™ for Starting Material

🔍 Detect Hidden Genomic Abnormalities

  • Identify balanced/unbalanced translocations, aneuploidy, and deletions with high specificity

  • Go beyond karyotyping with detection limits down to just a few kilobases

🧬 Ensure Genome Integrity Pre-Editing

  • Screen for baseline abnormalities before genome engineering begins

  • Reduce confounding factors in editing efficiency and outcome variability

📊 Population-Level Insight with Single-Cell Resolution

  • Understand heterogeneity within your starting material

  • Detect subclonal events that bulk sequencing averages out

⚠️ De-Risk Regulatory Pathways

  • Generate data suitable for regulatory submission, strengthening the rationale for cell selection and QC

  • Support risk assessment models with high-confidence genomic evidence


Integrated, Scalable, and Ready to Go

KROMASURE™ is optimized for interphase analysis, eliminating the need for metaphase prep and reducing turnaround time. It integrates seamlessly with other KROMATID solutions like PinPoint™ and InSite for a full-spectrum structural genomic view — from starting material to final product.


Better Inputs. Better Outcomes.

Starting material analysis is no longer optional — it’s a critical control point in the development of safe, effective cell and gene therapies. With the KROMASURE™ Platform, you gain unprecedented visibility into the genomic quality of your starting cells, empowering better science, smarter decisions, and stronger regulatory submissions.

Start smart. Start with KROMASURE™.

About the author

KROMATID Marketing