The Gold Standard for Non-Metaphase Genomic Insight
In the evolving landscape of cell and gene therapy, understanding where and how transgenes integrate into the genome is not just a scientific curiosity — it’s a regulatory and therapeutic imperative. While sequencing remains a cornerstone of genomic analysis, it often lacks the cellular granularity needed to fully characterize insertion events. That’s where KROMASURE PinPoint™ steps in.
PinPoint™ is a proprietary, high-throughput platform engineered to visualize and quantify transgene integrations at single-cell resolution — without the need for metaphase spreads. Designed to detect integration events as small as 2 kilobases, PinPoint sets a new benchmark for precision and throughput in genomic analysis.
Beyond the Average: Why Single-Cell Matters
Sequencing-based methods, while powerful, provide only population-level averages and algorithmic approximations of transgene copy number. But in gene-modified cells, integration events are rarely uniform. Some cells harbor one copy, others dozens — and this variability can impact efficacy, safety, and regulatory readiness.
PinPoint™ enables direct visualization of these events in thousands of individual nuclei, offering a level of insight that pooled sequencing simply can’t provide. With PinPoint, developers gain a true picture of how their platform behaves across an entire cell population.
Core Capabilities of PinPoint™
🧬 Characterize Small Target Integrations
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Detects integrations as small as 2–10 kb, allowing for precise localization of small vectors or editing events.
🔬 Single-Cell Resolution
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Measures modal distribution and copy number at the cell and chromosomal level.
🔢 Accurate Transgene Enumeration
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Quantifies the exact number of transgene copies in each nucleus, not just population estimates.
🧹 Deletion & Aneuploidy Detection
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Identifies deleted genetic material as well as monosomy and chromosome copy number variations — key factors in therapeutic risk profiling.
🧪 Flexible Sample Compatibility
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Works with hepatocytes and other interphase cells, eliminating the need for metaphase preparation.
Unmatched Insertional Copy Number Insight
PinPoint isn't just about detecting integration — it's about deeply understanding it. With its advanced capabilities, users can:
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Map integrations to specific chromosomes
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Analyze thousands of cells for statistical power and confidence
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Profile genomic risk with precision for regulatory compliance
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Understand variation across the entire population, not just the average
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Seamlessly integrate findings with KROMASURE™ InSite for orthogonal data interpretation
Applications Across the Gene Therapy Spectrum
Whether you’re working on viral vector integration, gene editing platform development, or regulatory submission support, KROMASURE PinPoint™ provides actionable insights across key application areas:
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✅ Measure Genomic Integrity
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✅ Assess Edit Integrity
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✅ Map Transgene Insertion
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✅ Support Regulatory Compliance
Why PinPoint™ Sets the Standard
In an industry where precision equals progress, KROMASURE PinPoint™ offers the most comprehensive and scalable solution for understanding transgene behavior at the cellular level. Whether you’re navigating discovery science or preparing for clinical submission, PinPoint provides the clarity you need to move forward with confidence.
Ready to see what your genome is really doing?
Contact KROMATID today to learn how PinPoint™ can elevate your integration analysis.