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    • FISH PROBES
      • HUMAN WHOLE CHROMOSOME PINPOINT FISH™ PAINT PROBES
      • SUBCENTROMERE PINPOINT FISH™ PROBES
      • SUBTELOMERE PINPOINT FISH™ PROBES
      • ONCOLOGY PINPOINT FISH™ PROBES
    • FISH KITS
      • TP53/CEP17 PINPOINT FISH™ PROBE
    • DGH™ KITS
      • dGH IN-SITE™ CAR-T PROBE KIT
      • dGH™ ASSAYS CELL PREP KIT
    • BUFFERS AND REAGENTS
  • SERVICES
    • PLASMID SERVICES
      • CUSTOM PLASMIDSNEW!
      • PLASMID PREPSNEW!
    • ASSAY SERVICES
      • dGH IN-SITE™ TARGETED ASSAY SERVICES
      • dGH SCREEN™ UNBIASED ASSAY SERVICES
      • PINPOINT FISH™ ASSAY SERVICES
    • RESEARCH SERVICES
      • CELL CULTURE SERVICES
      • G BANDING SERVICES
  • SUPPORT & FAQ’s
    • TECHNICAL INFORMATION
      • MICROSCOPE SPECIFICATIONS DATA SHEET
      • WATER BATH APPLICATION NOTES FOR PINPOINT FISH™ PROBES
      • MULTIPLEXING WITH PINPOINT FISH™ PROBES APPLICATION NOTES
      • FIRST TIME USERS APPLICATION NOTES FOR PINPOINT FISH™ PROBES
    • PRESENTATIONS & POSTERS
      • SCIENTIFIC PRESENTATIONS
      • POSTERS
    • FREQUENTLY ASKED QUESTIONS
  • ABOUT KROMATID
    • dGH™ TECHNOLOGY
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    • CAREERS
    • BLOG
    • KROMATID NEWS
  • PROJECT INQUIRY
  • PRODUCTS
    • FISH PROBES
      • HUMAN WHOLE CHROMOSOME PINPOINT FISH™ PAINT PROBES
      • SUBCENTROMERE PINPOINT FISH™ PROBES
      • SUBTELOMERE PINPOINT FISH™ PROBES
      • ONCOLOGY PINPOINT FISH™ PROBES
    • FISH KITS
      • TP53/CEP17 PINPOINT FISH™ PROBE
    • DGH™ KITS
      • dGH IN-SITE™ CAR-T PROBE KIT
      • dGH™ ASSAYS CELL PREP KIT
    • BUFFERS AND REAGENTS
  • SERVICES
    • PLASMID SERVICES
      • CUSTOM PLASMIDSNEW!
      • PLASMID PREPSNEW!
    • ASSAY SERVICES
      • dGH IN-SITE™ TARGETED ASSAY SERVICES
      • dGH SCREEN™ UNBIASED ASSAY SERVICES
      • PINPOINT FISH™ ASSAY SERVICES
    • RESEARCH SERVICES
      • CELL CULTURE SERVICES
      • G BANDING SERVICES
  • SUPPORT & FAQ’s
    • TECHNICAL INFORMATION
      • MICROSCOPE SPECIFICATIONS DATA SHEET
      • WATER BATH APPLICATION NOTES FOR PINPOINT FISH™ PROBES
      • MULTIPLEXING WITH PINPOINT FISH™ PROBES APPLICATION NOTES
      • FIRST TIME USERS APPLICATION NOTES FOR PINPOINT FISH™ PROBES
    • PRESENTATIONS & POSTERS
      • SCIENTIFIC PRESENTATIONS
      • POSTERS
    • FREQUENTLY ASKED QUESTIONS
  • ABOUT KROMATID
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  • 1/4 -Example of in-Site and SCREEN combination assay in unedited human fibroblast control line cell. Bracketing in-Site probes on chromosome 22 (circled) to identify insert target location and dGH SCREEN paints on chromosomes 1, 2, and 3 (pink). No insert would be expected in this control line
  • 2/4 - Example of a single cell from the same in-Site assay in an edited iPSC viewed in multiple fluorescence channels. Left image shows all fluorescence channels overlaid, middle shows yellow/insert probe channel, and right shows green/on-target bracketing probe channel. Multiple inserts are visible outside of the on-target bracketing probes, indicating high levels of off-target integrations in this cell.
  • 3/4 - Example of the same in-Site assay in an edited iPSC line cell with no off target insertions. Target site bracketing probes in green and insert probe in yellow zoomed in and expanded on the right. Results from this sample indicated that 6% of cells had no off-target insertions.
  • 4/4 - Image from a dGH in-Site assay in polyploid, dual-edited, HEK-293FT cells demonstrating both normal and rearranged edit site structures. At the normal edit sites (A), both sets of in-Site probes are located on the same side of the chromosome. An inversion at the edit site is also visible (B), with one portion of the in-Site probe switching orientations and showing up on the opposite chromatid from the other portions of the in-Site probe. A partial deletion at one edit site is also observed (C), with the red portion of the in-Site probes missing.
AvadaSliderMary Lukens2023-03-16T02:21:13-06:00

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