We will grow primary or established cells at various scales. Starting from your cell lines or blood provided to us, we will handle your cell line growth, expansion, isolation and quality control. No capital costs or extra time on your end. Our Cell services will include recovery of the culture from cryopreservation, assessment of the viability of your cells, at our above 50%, doubling of beginning cell count which will influence the yields that are determined by ending cell viability and assessment. With KromaTiD’s Cell Culture Services you can focus on discovering what matters most in your research, finding the answers to the most important questions!
We offer ISPC cell culture services, T-Cell culture services, adherent and suspended cell line culture services, Whole Blood Services, Growth and Assessment Viability services. We also provide DNA isolation cell, mycoplasma testing and metaphase preparation services.
Metaphase preparation is used to determine the instability of a chromosome and is especially important in Cytogenetics. It can be an art form in and of itself. This first step is harvesting the chromosomes, your cells must be healthy when harvesting. Next, the cell cycle is arrested, using a Colcemid agent. Cells are then harvested and fixed. Humidity is optimized at this stage. Once the slides are dried, they are ready for other applications such as immunostaining.
Cells are broken to release the DNA, separated from proteins and other cellular debris, the DNA is precipitated cleaned and the quality of the DNA is confirmed.
Cell Quality Control
Cell QC is the identification and counting of the cells, including the discrimination between the dead and the living cells. Cell QC is an integral component of any Quality Control procedures for any cell-based manufacturer.
Cell viability assays use a variety of markers as indicators of metabolically active (living) cells. Examples of markers commonly used include measuring ATP levels, measuring the ability to reduce a substrate, and detecting enzymatic/protease activities unique to living cells.
Growth Rate Determination
Determining growth rate is important as it represents the dynamic behavior of the microorganisms. It is important for controlling your cells, based on the appropriate methods an estimation of your growth rate can be determined. This precise cellular reproduction also known as Mitosis, is important to understanding the individual study.
The only way to confirm mycoplasma contamination is by routine testing using one or more special techniques, including direct growth on broth/agar, specific DNA staining, PCR, ELISA, RNA labeling and enzymatic procedures. The key to mycoplasma testing is frequency, every 1-2 months is important, and cells should not contain any antibiotics.