Pinpoint FISH™ Probes
Bioinformatically designed, synthetic oligonucleotide probes provide unparalleled presion, with their high signal-to-noise ratio and the lowest background.
Experience the next generation of FISH assays with Pinpoint FISH™, a groundbreaking synthetic oligonucleotide probe design approach which offers unparalleled precision, and the industry’s lowest limit of detection.
Control Probe Catalog:
Our catalog of subcentromere, subtelomere and paints can be used as control probes combined with your gene targets, in multiplexed assays with 2 or more probes. Assess unique regions or targets, especially if relatively precise breakpoints are necessary for break apart or fusion assay designs.
Gene Probe Catalog:
Gene probes are used to identify specific genetic sequences within a DNA sample, enabling researchers to pinpoint and study particular genes or mutations. They play a crucial role in various applications, such as diagnosing genetic disorders and studying gene expression in molecular biology research.
Custom Pinpoint FISH™ Probes:
Design custom Pinpoint FISH™ probes for novel targets and assay configurations not possible with conventional BAC-derived FISH probes. Custom Pinpoint FISH probes are a straightforward and cost-effective way to assay unique regions or targets, especially if relatively precise breakpoints are necessary for break apart or fusion assay designs.
- Target can be any published genome: human, mouse, rat, CHO, canine, non-human primates, viral vectors, transgenes.
- Probes for targets smaller than 5 kilobases are possible, depending on the availability of unique sequences in the locus of interest.
- Sample type can also affect LOD. Generally, the highest resolution is possible with metaphase samples, followed by dissociated cells and then tissues.
Pinpoint FISH probes are available for Research Use Only (RUO), although probes produced as Analyte Specific Reagents (ASR) can be developed and supplied for use in CLIA-compliant testing labs.
Versatile Custom Designs:
- Synthetic probes vs. cloned DNA (BAC FISH)
- Design against any published genome
- Detect smaller mutations and mutations with variable breakpoints
- Multiplex to generate custom data sets
- Suitable for use in GLP and CLIA testing (with appropriate development and QMS)
Key Advantages of Pinpoint FISH™
Complete Assay Solution: Our services offer a comprehensive solution, including assay execution, imaging, and scoring.
Versatile Sample Compatibility: Pinpoint FISH™ is compatible with both interphase and metaphase samples, enabling data gathering in a wide range of research scenarios.
Applicable Genomes: Probes can be designed against any published mammalian genome, as well as sequence files provided to KromaTiD.
Tailored Multiplexing: Bioinformatic screening of the oligonucleotide sequences used for Pinpoint FISH™ probes enables researchers to combine probes freely and generate unique data sets.
High Resolution Custom Probes: Custom probes can target loci as small as a few kilobases, thanks to the high signal-to-noise ratio of synthetic oligonucleotide probes.
Reliable Hybridization: Count on Pinpoint FISH™ for consistent hybridization quality that minimizes variability and guarantees dependable results.
Applications Include:
Targeted DNA Sequences: Oncology DNA probes are designed to bind to specific DNA sequences that are known to be associated with cancer. Detect mutations, gene rearrangements, or amplifications that play a role in the development and progression of cancer with high signal to noise ratio. Making detection of these DNA sequences simple.
Detection of Genetic Abnormalities: Whether using in situ hybridization (ISH) or fluorescence in situ hybridization (FISH) methods, you can easily identify genetic abnormalities, such as mutations in oncogenes or tumor suppressor genes, which are commonly found in cancer cells. An important tool for aiding in diagnostics of specific types of cancer.
In Situ Hybridization (ISH): Oncology DNA probes are commonly used in techniques like in situ hybridization (ISH), including fluorescence in situ hybridization (FISH) and chromogenic in situ hybridization (CISH). ISH methods allow for the visualization of specific DNA sequences within the cell or tissue sample, enabling the detection of genetic abnormalities directly in patient samples.
Customized for Target Genes: Our Pinpoint FISH™ DNA probes can be customized for different types of specific genetic alterations associated with those cancers. For example, there are probes designed for relevant genetic markers such as EFGR, TP53, and ALK.
Research and Diagnosis: Pinpoint FISH probes are available for Research Use Only, although probes produced as Analyte Specific Reagents can be developed and supplied for use in CLIA compliant testing labs. These probes help scientists better understand the molecular mechanisms of cancer.
Pinpoint FISH™ DNA probes are powerful tools that aid in the diagnosis and treatment of cancer to identify genetic alterations associated with the disease. Their use is essential in the ongoing effort to develop more effective cancer therapies.
Contact Us Today!
Visit our catalog pages, or request a quote for a custom probe.