Radiation Biodosimetry

Visualize and Quantify Inversions with dGH SCREEN™

dGH SCREEN™, the original KromaTiD assay, was utilized by NASA in the “Twin’s Study” to measure DNA damage from deep space radiation. NASA’s first foray into “omics” research represented a unique opportunity to develop a next-generation personalized medicine approach to monitoring the health of astronauts.

Today our dGH SCREEN™ platform is still being used by NASA to assess structural rearrangements in the genomes of their best and brightest. dGH SCREEN™ technology is a unique single stranded design allows researchers to visualize and quantify inversions as an inherently more stable biodosimeter than dicentrics, which enables them to obtain data much longer after a radiation event. In addition to greater stability, inversions occur with lower background frequency than other symmetrical aberrations such as translocations, providing fewer false positives and more reliable data.

Fluorescent Paints Spot DNA Damage from Radiation, Gene Editing →

Learn More About dGH SCREEN™ →

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1/5 - Data from 6 undiagnosed disease patients and 5 of their family members were assessed with dGH paints through the Undiagnosed Disease Consortium. Here, a cell from one of the patients demonstrates a pericentric inversion on chromosome 2 (circled).
2/5 - A cell from another patient from the Undiagnosed Disease Consortium demonstrating a paracentric inversion on chromosome 2 (circled).
3/5 - De novo discovery of a previously unsuspected large inversion (circled) found in roughly 40% of cells on chromosome 2 in an undiagnosed disease patient.
4/5 - Follow up assay in which a probe ladder was designed for the antisense strand, and used in combination with chromatid 2 paint to narrow down the breakpoint region locations. This image demonstrates a normal cell without rearrangements.
5/5 - Antisense ladder and chromatid 2 paint combination assay demonstrating an inversion between the ladder and paint (circled).

Radiation Biodosimetry

Visualize and Quantify Inversions with dGH SCREEN™

Fluorescent Paints Spot DNA Damage from Radiation, Gene Editing →

Learn More About dGH SCREEN™ →

1/5 - Data from 6 undiagnosed disease patients and 5 of their family members were assessed with dGH paints through the Undiagnosed Disease Consortium. Here, a cell from one of the patients demonstrates a pericentric inversion on chromosome 2 (circled).

2/5 - A cell from another patient from the Undiagnosed Disease Consortium demonstrating a paracentric inversion on chromosome 2 (circled).

3/5 - De novo discovery of a previously unsuspected large inversion (circled) found in roughly 40% of cells on chromosome 2 in an undiagnosed disease patient.

4/5 - Follow up assay in which a probe ladder was designed for the antisense strand, and used in combination with chromatid 2 paint to narrow down the breakpoint region locations. This image demonstrates a normal cell without rearrangements.

5/5 - Antisense ladder and chromatid 2 paint combination assay demonstrating an inversion between the ladder and paint (circled).

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