dGH in-Site™

Examine Edits One Cell at a Time

dGH in-Site™ gives researchers the ability to directly visualize and characterize rearrangements at their edit sites. Using synthetic oligo probes and repeat-free bioinformatic design, dGH in-Site is capable of tracking edit site translocations, inversions, copy number variants, viral integrations and other complex rearrangements with an ≈2kb resolution.

As an orthogonal analysis to PCR/sequencing techniques, dGH in-Site™ assays enable, through direct visualization, definitive measurement of the presence or absence of inserts. This includes genome-wide distribution and orientation of transgenes or inserted segments. When used in combination with our targeted probes and chromosome paints, dGH in-Site™ assays can provide quantitative, single cell on- vs. off-target outcomes of the delivery and editing process, and can be used to track the stability of the edited genome in both clonal isolates and non-clonal populations.

Concerns over a safe edit site? dGH in-Site™ allows researchers to examine their edits one cell at a time without having to worry about PCR biases or misrepresentative sequencing averages from heterogeneous cell populations. An orthogonal and complementary technique dGH in-Site™ to PCR/sequencing techniques helps provide the most comprehensive data available.

1/4 -Example of in-Site and SCREEN combination assay in unedited human fibroblast control line cell. Bracketing in-Site probes on chromosome 22 (circled) to identify insert target location and dGH SCREEN paints on chromosomes 1, 2, and 3 (pink). No insert would be expected in this control line
2/4 - Example of a single cell from the same in-Site assay in an edited iPSC viewed in multiple fluorescence channels. Left image shows all fluorescence channels overlaid, middle shows yellow/insert probe channel, and right shows green/on-target bracketing probe channel. Multiple inserts are visible outside of the on-target bracketing probes, indicating high levels of off-target integrations in this cell.
3/4 - Example of the same in-Site assay in an edited iPSC line cell with no off target insertions. Target site bracketing probes in green and insert probe in yellow zoomed in and expanded on the right. Results from this sample indicated that 6% of cells had no off-target insertions.
4/4 - Image from a dGH in-Site assay in polyploid, dual-edited, HEK-293FT cells demonstrating both normal and rearranged edit site structures. At the normal edit sites (A), both sets of in-Site probes are located on the same side of the chromosome. An inversion at the edit site is also visible (B), with one portion of the in-Site probe switching orientations and showing up on the opposite chromatid from the other portions of the in-Site probe. A partial deletion at one edit site is also observed (C), with the red portion of the in-Site probes missing.

Ordering Information

Products	List Price
Non-refundable set-up fee (when applicable)	Project Specific
Assay, execution, imaging, and scoring (1 Probe)	$3,220/sample
Assay, execution, imaging, and scoring: Additional Probes	$1,000/probe
Custom Probes (Design and Verification)	$5,000/probe
Standard Probe Production	$1,000/probe
Culture Development	$500/sample
Cell Culture + Harvest	$500/sample

Inquire

INQUIRE

dGH in-Site™

Examine Edits One Cell at a Time

1/4 -Example of in-Site and SCREEN combination assay in unedited human fibroblast control line cell. Bracketing in-Site probes on chromosome 22 (circled) to identify insert target location and dGH SCREEN paints on chromosomes 1, 2, and 3 (pink). No insert would be expected in this control line

3/4 - Example of the same in-Site assay in an edited iPSC line cell with no off target insertions. Target site bracketing probes in green and insert probe in yellow zoomed in and expanded on the right. Results from this sample indicated that 6% of cells had no off-target insertions.

Ordering Information

Products

List Price

Inquire

RESOURCES

PRODUCTS

SERVICES

Title