Pinpoint FISH™ Assays

High-resolution FISH assays and custom probes for any published genome.

Above Fig.: Custom Pinpoint FISH™ probe designed for Chromosome 1 Ladder.

Experience the Next Generation of FISH Assays with Pinpoint FISH™

Optimize your FISH assays with our bioinformatically-designed, synthetic Pinpoint FISH™ custom probes and assay services.  

Custom Pinpoint FISH probes are a straightforward and cost-effective way to assess unique regions or targets. 

Key Advantages of Pinpoint FISH™ Services 

  • Comprehensive: Expert assay design, execution, and analysis. 
  • Cell culture service for both interphase and metaphase analysis. 
  • Pinpoint FISH Probes  
    • Custom designs 
    • Available for any published genome: Human, mouse, rat, CHO, canine, non-human primates, viral vectors, and transgenes. 
    • High-resolution custom probes: Designed for your custom target. 
  • Broad compatibility: Tailored multiplexing and reliable hybridization. 
  • Visualization of small targets. 

Contact us to schedule a meeting to discuss how we can assist you with your FISH assays and custom probe design for your research needs! 

Above Fig.: Polyploid rat cell with two HIV sequence integration signals (red) and four control signals (yellow).

Non-human species probes inject a layer of additional complexity in humanized model systems.

KromaTiD hasproven experience in designing and producing custom probes for any sequenced species. Our expert experience in culture, harvest, slide preparation and hybridization of non-human lines provide you confidence in your assay results.  Rodent models play a critical role in research. Here, two custom probes were created for a project involving rats, one targeting an integrated HIV sequence (red), and the other targeting TP53 (yellow) as a control. Two integrations are detected in this polyploid, metaphase spread.

Above Fig.: Edited T cell hybridized with custom probes labeling loci of interest, two of which are involved in a translocation. (circles) The top circle features a dicentric chromosome.

High-resolution is critical when assaying gene editing outcomes.

KromaTiD designs custom probes to detect structural rearrangements at key gene editing loci, as in this image of a triple-edited T cell. The two circled objects each contain translocation derivatives, with the top one representing a dicentric chromosome. KromaTiD can design assay components to target edit sites, transgenes, and homology markers, depending on the strategy and dataset required. Designs can target loci as small as 2 kb.

Above Fig.: Breast cancer tissue was probed with HER2 Pinpoint FISH™ probe (red) and analyzed for amplification. (A) Normal (non-amplified) HER2 signals (B-C) Examples of cells with HER2 amplification.

Custom Tissue Assays

Accurate HER2 testing can help reduce unnecessary costs and treatment side-effects, and have important prognostic implications, which means HER2-related research has a high potential pay-off. Assessment of the HER2 amplification status of a sample can be very nuanced. It is essential that FISH probes perform with a high signal-to-noise ratio, even within the heterogeneous setting of a solid tissue sample, where the outcome is influenced by factors like fixation chemistry and time, as well as subsequent digestion and permeabilization steps. Your samples are precious, as are your project’s resources.

Order Below
Catalog Number
Services
Cost
PPF-010 Standard Probe Production: Design and verification of standard probes $1,444

PPF-011

Custom Probe Production: Design and verification of custom probes Inquire
PPF-002 T-Cell Culture Development: Thaw, recovery, and harvest optimization $1,209
PPF-014 T Cell Metaphase Prep and Harvest $1,209
PPF-007 IPSC Cell Culture Development: Thaw, recovery, and harvest optimization $1,344
PPF-012 IPSC Metaphase Prep and Harvest $1,344
PPF-009 Whole Blood Culture Development: Thaw, recovery, and harvest optimization $739
PPF-013 Whole Blood Metaphase Prep and Harvest $739
PPF-017 NK Cell Culture Development: Thaw, recovery, and harvest optimization $1,209
PPF-016 NK Cells Metaphase Prep and Harvest $1,209
PPF-006 1 probe assay, 200 cells counted per sample, interphase. Execution and Analysis $3,816
PPF-015 1 probe assay, 500 cells counted per sample, interphase. Execution and Analysis $4,543
PPF-020  2 probe assay, 20 cells counted per sample, interphase. Assay Execution and Analysis $1,441
PPF-005  2 probe assay, 200 cells counted per sample, interphase. Assay Execution and Analysis $5,001
PPF-018  2 probe assay, 500 cells counted per sample, interphase. Assay Execution and Analysis $5,952
PPF-004 3 probe assay, 200 cells counted per sample, interphase. Assay Execution and Analysis $6,187
PPF-019 3 probe assay, 500 cells counted per sample, interphase. Assay Execution and Analysis $7,380
PPF-003 4 probe assay, 200 cells counted per sample, interphase. Assay Execution and Analysis $8,425
PPF-AUT Assay Development and Automation $1,125
PPF-DEV Assay Development and Qualification $5,375
PPF-BUF-001 FISH Buffers – 10 tests $6