Pinpoint FISH TP53/CEP17 probe kit is an oligonucleotide-based FISH assay designed to provide the highest resolution, lowest background, and lowest limit of detection available. By modifying the hybridization conditions and eliminating strand degradation steps.
The TP53/CEP17 probe kit provides 3x the relative signal; 3x the resolution and 6x the signal-to-noise ratio; 50% lower background, compared to similar oligonucleotide assays. Pinpoint probes can be designed for a wider range of targets and target sizes.
With KromaTiD’s probe kit you can easily detect mutations with variable breakpoints free of repetitive sequences, and no blocking required, providing Robust performance and consistent hybridization quality.
P53, 17 13. Red
D17Z1, 17q11.1-q11.2, Green
TP53 probe mix consists of a 167kb probe, labelled in red that covers the whole TP53 gene and the flanking regions. The probe mix also contains a control probe for the 17 centromere (D17Z1) that is labelled in green.
The Pinpoint FISH TP53/CEP 17 FISH Probe Kit is intended to detect the copy number of the LSI TP53 probe target located at chromosome 17q11.1 and of the CEP 17 probe target located at the centromere of chromosome 17.
The approximately 167kb TP53 probe contains the complete TP53 gene and is located at 17p13. The CEP 17 probe is a control probe which hybridizes to the centromere region of chromosome 17q11.1-11.2.