Plasmid DNA Manufacturing
From research-grade to cGMP DNA plasmids, KromaTiD’s state of the art facility for plasmid manufacturing can provide high throughput, a cGMP suite and the range of plasmid DNA purity level and batch size to fit any scale project.
Our team of experts provides the highest quality DNA plasmids for preclinical research studies through IND filing, and for clinical trials through commercial manufacturing.
We Scale With You
A comprehensive, secure path for your plasmid development and testing
Key Features
- From RUO to Commercial stage
- Three purity grades: research, transfection and endotoxin-free
- pDNA formats accepted: frozen glycerol stock or stabs for cultures
KromaTiD pDNA Manufacturing Supports a Variety of Applications and Scale
Applications
- Viral Vectors (AAV, Lentivirus)
- DNA Vaccines
- CAR-T Cell Therapy
- Genome Editing
- Cloning & DNA Synthesis
- High-Throughput Screening
- mRNA Production
- Transfection
|
pDNA Grade |
Scale |
Production Mode |
TAT |
|---|---|---|---|
| Research | 100µg-2.5mg | Isolated, controlled production | 5-10 days |
| Transfection | 100µg-1g | Isolated, controlled production with transfection-grade purification | ≤2.5mg, 5-10 days >2.5mg, Inquire |
| Low Endotoxin | 100µg-1g | Isolated, controlled production with low endotoxin-grade purification | ≤2.5mg, 5-10 days >2.5mg, Inquire |
| Pre-GMP | ≥50mg | Dedicated, segregated non-cGMP lab | ~6 weeks |
| cGMP | ≥50mg | cGMP-certified production suites | Inquire |
Download Product Data Sheet for pricing and product numbers:
Plasmid Service Overview
|
Service Provided |
Research |
Pre-GMP |
cGMP |
|---|---|---|---|
| Cloning, synthesis, scale-up and manufacturing | ✓ | ✓ | ✓ |
| Multiple production scales (shake flask through fermenters) |
✓ | ✓ | ✓ |
| Proven manufacturing and purification processes for high yield and recovery | ✓ | ✓ | ✓ |
| Standardized manufacturing platforms | ✓ | ✓ | |
| Upstream and downstream process development | ✓ | ✓ | |
| Production in segregated and dedicated space | ✓ | ✓ | |
| Master Batch Records | ✓ | ✓ | |
| Document support for IND filing; Stability studies, product release tests |
✓ | ✓ | |
| Manufacturing in cGMP suite with upgraded air handling | ✓ | ||
| Complete QC monitoring throughout production process | ✓ | ||
| QA oversight for production | ✓ |
Quality Control Methods
|
QC Metric |
Specification |
Measurement Method |
|---|---|---|
| A260/280 | 1.8–2.0 | 1.8–2.0 |
| Appearance | Clear | Visual inspection |
| Concentration | On request, standard is 0.25 mg/mL | UV absorption |
| Supercoil content | >80% | Agarose gel |
| Residual RNA | Not visible | Agarose gel |
| Genomic DNA | Not visible | Agarose gel |
| Restriction digest | Matches reference | Enzymatic digestion and gel electrophoresis |
| Endotoxin assay | LAL assay | < 0.1 EU/µg plasmid DNA |
| Bioburden assay | No growth | Agar plate, 48 hours |
Plasmid Manufacturing Pricing
|
Catalog Number |
Product Description |
TAT |
List Price |
|---|---|---|---|
| KPM-001 | Research-Grade up to 100ug yield | 5-10 days | $437.50 |
| KPM-002 | Research-Grade up to 200ug yield | 5-10 days | $443.10 |
| KPM-003 | Research-Grade up to 500ug yield | 5-10 days | $448.35 |
| KPM-004 | Research-Grade up to 1mg yield | 5-10 days | $458.85 |
| KPM-005 | Research-Grade up to 2.5mg yield | 5-10 days | $511.70 |
| KPM-006 | Research-Grade >2.5mg yield | Inquire | Inquire |
| KPM-007 | Transfection-Grade up to 100ug yield | 5-10 days | $439.43 |
| KPM-008 | Transfection-Grade up to 200ug yield | 5-10 days | $457.10 |
| KPM-009 | Transfection-Grade up to 500ug yield | 5-10 days | $461.48 |
| KPM-010 | Transfection-Grade up to 1mg yield | 5-10 days | $669.10 |
| KPM-011 | Transfection-Grade up to 2.5mg yield | 5-10 days | $802.55 |
| KPM-012 | Transfection-Grade >2.5mg yield | Inquire | Inquire |
| KPM-013 | Low Endotoxin-Grade up to 100ug yield | 5-10 days | $449.50 |
| KPM-014 | Low Endotoxin-Grade up to 200ug yield | 5-10 days | $467.25 |
| KPM-015 | Low Endotoxin-Grade up to 500ug yield | 5-10 days | $474.25 |
| KPM-016 | Low Endotoxin-Grade up to 1mg yield | 5-10 days | $735.00 |
| KPM-017 | Low Endotoxin-Grade up to 2.5mg yield | 5-10 days | $861.00 |
| KPM-018 | Low Endotoxin-Grade >2.5mg yield | Inquire | Inquire |
| KPM-019 | Pre-GMP Grade up to 50mg yield | -6weeks | Inquire |
| KPM-020 | cGMP Grade 100mg to 5g yield | Inquire | Inquire |
| KPM-021 | DNA Synthesis per DNA basepair | 4-8 weeks | .78t/base pair |
| KPM-022 | Sanger Sequencing Verification | 5 days | Inquire |
| KPM-023 | NGS Sequencing Verification | 5 days | $170.00 |