Plasmid DNA Manufacturing

From research-grade to cGMP DNA plasmids, KromaTiD’s state of the art facility for plasmid manufacturing can provide high throughput, a cGMP suite and the range of plasmid DNA purity level and batch size to fit any scale project.

Our team of experts provides the highest quality DNA plasmids for preclinical research studies through IND filing, and for clinical trials through commercial manufacturing.

We Scale With You

we scale with you

 

A comprehensive, secure path for your plasmid development and testing

Key Features

  • From RUO to Commercial stage
  • Three purity grades: research, transfection and endotoxin-free
  • pDNA formats accepted: frozen glycerol stock or stabs for cultures

KromaTiD pDNA Manufacturing Supports a Variety of Applications and Scale

Applications

  • Viral Vectors (AAV, Lentivirus)
  • DNA Vaccines
  • CAR-T Cell Therapy
  • Genome Editing
  • Cloning & DNA Synthesis
  • High-Throughput Screening
  • mRNA Production
  • Transfection

pDNA Grade

Scale

Production Mode

TAT

Research 100µg-2.5mg Isolated, controlled production 5-10 days
Transfection 100µg-1g Isolated, controlled production with transfection-grade purification ≤2.5mg, 5-10 days
>2.5mg, Inquire
Low Endotoxin 100µg-1g Isolated, controlled production with low endotoxin-grade purification ≤2.5mg, 5-10 days
>2.5mg, Inquire
Pre-GMP ≥50mg Dedicated, segregated non-cGMP lab ~6 weeks
cGMP ≥50mg cGMP-certified production suites Inquire

Download Product Data Sheet for pricing and product numbers:

PLASMID MANUFACTURING DATA SHEET

Plasmid Service Overview

Service Provided

Research

Pre-GMP

cGMP

Cloning, synthesis, scale-up and manufacturing
Multiple production scales
(shake flask through fermenters)
Proven manufacturing and purification processes for high yield and recovery
Standardized manufacturing platforms
Upstream and downstream process development
Production in segregated and dedicated space
Master Batch Records
Document support for IND filing;
Stability studies, product release tests
Manufacturing in cGMP suite with upgraded air handling
Complete QC monitoring throughout production process
QA oversight for production

Quality Control Methods

QC Metric

Specification

Measurement Method

A260/280 1.8–2.0 1.8–2.0
Appearance Clear Visual inspection
Concentration On request, standard is 0.25 mg/mL UV absorption
Supercoil content >80% Agarose gel
Residual RNA Not visible Agarose gel
Genomic DNA Not visible Agarose gel
Restriction digest Matches reference Enzymatic digestion and gel electrophoresis
Endotoxin assay LAL assay < 0.1 EU/µg plasmid DNA
Bioburden assay No growth Agar plate, 48 hours

Plasmid Manufacturing Pricing

Catalog Number

Product Description

TAT

List Price

KPM-001 Research-Grade up to 100ug yield 5-10 days $437.50
KPM-002 Research-Grade up to 200ug yield 5-10 days $443.10
KPM-003 Research-Grade up to 500ug yield 5-10 days $448.35
KPM-004 Research-Grade up to 1mg yield 5-10 days $458.85
KPM-005 Research-Grade up to 2.5mg yield 5-10 days $511.70
KPM-006 Research-Grade >2.5mg yield Inquire Inquire
KPM-007 Transfection-Grade up to 100ug yield 5-10 days $439.43
KPM-008 Transfection-Grade up to 200ug yield 5-10 days $457.10
KPM-009 Transfection-Grade up to 500ug yield 5-10 days $461.48
KPM-010 Transfection-Grade up to 1mg yield 5-10 days $669.10
KPM-011 Transfection-Grade up to 2.5mg yield 5-10 days $802.55
KPM-012 Transfection-Grade >2.5mg yield Inquire Inquire
KPM-013 Low Endotoxin-Grade up to 100ug yield 5-10 days $449.50
KPM-014 Low Endotoxin-Grade up to 200ug yield 5-10 days $467.25
KPM-015 Low Endotoxin-Grade up to 500ug yield 5-10 days $474.25
KPM-016 Low Endotoxin-Grade up to 1mg yield 5-10 days $735.00
KPM-017 Low Endotoxin-Grade up to 2.5mg yield 5-10 days $861.00
KPM-018 Low Endotoxin-Grade >2.5mg yield Inquire Inquire
KPM-019 Pre-GMP Grade up to 50mg yield -6weeks Inquire
KPM-020 cGMP Grade 100mg to 5g yield Inquire Inquire
KPM-021 DNA Synthesis per DNA basepair 4-8 weeks .78t/base pair
KPM-022 Sanger Sequencing Verification 5 days Inquire
KPM-023 NGS Sequencing Verification 5 days $170.00

Inquire

INQUIRE

RESOURCES

Buffers and Reagents
Plasmid Manufacturing Overview
PLASMID MANUFACTURING DATA SHEET

FISH PROBES AND SERVICES

Centromere Pinpoint FISH™ Probes
subTelomere Pinpoint FISH™ Probes
ONCOLOGY PINPOINT FISH™ PROBES
PINPOINT FISH™ TP53/CEP17 KIT
HUMAN WHOLE CHROMOSOME PINPOINT FISH™ PAINT PROBES
PINPOINT FISH™ ASSAY SERVICES

GENOMIC ASSAY PRODUCTS AND SERVICES

dGH™ CELL PREP KIT
DGH SCREEN™ UNBIASED ASSAY SERVICES
DGH IN-SITE™ TARGETED ASSAY SERVICES

RESEARCH SERVICES

Cell Culture Services
G-Banding

Cell Culture Protocol Video